Changes in Height and Body Weight Within a Time Period of 35 Years in Males of the Same Age / Cambios en la Altura y el Peso Corporal en un Período de 35 Años en Hombres de la Misma Edad

SUMMARY: Height and body weight measurements are among the most important anthropometric variables when assessing a population's growth, development and body composition. This study aimed to evaluate the height and body weight variability of male entities aged 17-18 years within 35 years. This goal was realized by comparing the height and body weight of the population of the same gender and age in three different time studies. The descriptive statistical parameters and T-test for independent groups show systematic and significant differences in measured variables between three measurements in different timelines. Both in body height and body weight, from measurement to measurement, significant systematic and statistically significant differences (p<0.01) have been identified (1985: BH= 172.8cm, BW= 61.7kg; 2004: BH=176.8 cm, BW=66.9 kg; 2019: BH=178.5 cm, BW=72 kg). The results of this study prove that the change in the socio-economic status of a population over a period time of 35 years can significantly affect the growth and development of children/adolescents.

Las medidas de altura y peso corporal se encuentran entre las variables antropométricas más importantes a la hora de evaluar el crecimiento, el desarrollo y la composición corporal de una población. Este estudio tuvo como objetivo evaluar la variabilidad de la altura y el peso corporal de entidades masculinas de 17 a 18 años dentro de 35 años. Este objetivo se logró comparando la altura y el peso corporal de la población del mismo sexo y edad en tres estudios temporales diferentes. Los parámetros estadísticos descriptivos y la prueba T para grupos independientes muestran diferencias sistemáticas y significativas en las variables medidas entre tres mediciones en diferentes líneas de tiempo. Tanto en la altura como en el peso corporal, de medición en medición, se han identificado diferencias significativas sistemáticas y estadísticamente significativas (p<0,01) (1985: BH= 172,8 cm, BW= 61,7 kg; 2004: BH=176,8 cm, BW=66,9 kg; 2019: BH=178,5 cm, BW=72 kg). Los resultados de este estudio demuestran que el cambio en el estatus socioeconómico de una población durante un período de 35 años puede afectar significativamente el crecimiento y desarrollo de niños/ adolescentes.

Cardiovascular Fitness in Normal Weight and Obese Children and Adolescents - A Systematic Review of Studies Published After 2000s / Condición Cardiovascular en Niños y Adolescentes con Peso Normal y Obesos: Una Revisión Sistemática de Estudios Publicados Después de la Década del 2000

SUMMARY: The primary aim was to gather available data published after the 2000s, on cardiovascular fitness in normal weight and obese children and adolescents. Based on the data, the secondary aims were to identify the most used outcomes and to determine the differences in cardiovascular fitness in the mentioned populations. Following PRISMA recommendations, multiple databases were searched: Google Scholar, PubMed, Cochrane Library, ProQuest, and Research Gate, with additional inclusion criteria: original study published in English, normal weight and obese children and adolescents as participant sample, studies that have assessed cardiovascular fitness parameters, and studies with participant's nutrition state. A total of 19 studies, with a total of 4,988 included participants (both obese and normal weight) were identified, with the most common participants have presented better results in terms of BMI, BF%, VO2max, VO2peak and METs, while the HR values are inconsistently presented. Despite deficiencies the study deficiency in the last two decades, there are differences in the mentioned populations. Further studies should focus on including the technology that reaches teens and families for overweight and obesity prevention and advancements in standard measurements for juvenile overweight and obesity, as well as for the creation, adaption, and validation of measuring instruments. As good framework for future directions, there is a need for more concise and unified measurements of cardiovascular fitness parameters in normally weight and obese children and adolescents.

El objetivo principal fue recopilar datos disponibles publicados después de la década del 2000 sobre la condición cardiovascular en niños y adolescentes con peso normal y obesos. En base a estos datos, los objetivos secundarios fueron identificar los resultados más utilizados y determinar las diferencias en la condición cardiovascular en las poblaciones mencionadas. Siguiendo las recomendaciones de PRISMA, se realizaron búsquedas en múltiples bases de datos: Google Scholar, PubMed, Cochrane Library, ProQuest y Research Gate, con criterios de inclusión adicionales: estudio original publicado en inglés, niños y adolescentes con peso normal y obesidad como muestra participante, estudios que hayan evaluado enfermedades cardiovasculares, parámetros de condición física y estudios con el estado nutricional de los participantes. Se identificaron un total de 19 estudios, con un total de 4.988 participantes incluidos (tanto obesos como con peso normal), siendo las variables más comunes: IMC, %BF, VO2máx, VO2pico, FC, PAD, PAS y MET. Generalmente, los participantes con peso normal han presentado mejores resultados en términos de IMC, %BF, VO2máx, VO2peak y MET, mientras que los valores de FC se presentan de manera inconsistente. Independientemente de la deficiencia de estudios en las últimas dos décadas, existen diferencias en las poblaciones mencionadas. Estudios futuros deberían centrarse en incluir tecnología que llegue a adolescentes y familias para la prevención del sobrepeso y la obesidad y avances en las mediciones estándar del sobrepeso y la obesidad juvenil, así como para la creación, adaptación y validación de instrumentos de medición. Como buen marco para direcciones futuras, se necesitan mediciones más concisas y unificadas de los parámetros de la condición cardiovascular en niños y adolescentes con peso normal y obesos.

Liver Size and its Correlation with Anthropometric Parameters and Age / El Tamaño del Hígado y su Correlación con los Parámetros Antropométricos y la Edad

SUMMARY: The liver has over 500 physiological and biochemical roles in our organism so checking of liver size and function is a part of every clinical examination. Aim of our research was to estimate liver size on computed tomography (CT) of the abdomen images and to determinate relations between liver dimensions and anthropometric parameters. The research included 99 patients, 49 men and 50 women, who were referred for CT of abdomen. We measured body height (BH) and body mass (BM), and calculated body mass index (BMI) and body surface area (BSA). Also, on CT images we measured anteroposterior (AP), laterolateral (LL) and two craniocaudal liver diameters (one at the level of midclavicular line - CCmcl, and the other was maximal - CCmax). Liver volume (LV) was calculated with formula. Our results showed that AP diameter positively correlated with BSA (r=0.30) in women. LL diameter positively correlated with BH (r=0.43), and BSA (0.31) in men. CCmcl diameter positively correlated with BH (r=0.33), BM (r=0.31), and BSA (r=0.34) in men, while in women it correlated only with BH (r=0.38). CCmax diameter positively correlated with BH (r=0.33) and BSA (r=0.33) in men. LV positively correlated with BH and BSA in both men (r=0.36, r=0.33, respectively) and women (r=0.42, r=0.31, respectively), and in men also with BM (r=0.34). LL, CCmcl, CCmax, and LV negatively correlated with aging in both sexes After the age of 60, there was a decrease in size of LL, CC diameters, as well as in LV. We concluded that liver dimensions decrease with aging, regardless of sex at the expanse of LL and CC diameters which are related to the size of body parameters, so that for a precise evaluation of liver size all three diameters should be measured, LV as well as BH, BM, and BSA.

El hígado desempeña más de 500 funciones fisiológicas y bioquímicas en nuestro organismo, por lo que comprobar el tamaño y la función de este órgano es parte de cada examen clínico. El objetivo de nuestra investigación fue estimar el tamaño del hígado mediante tomografía computarizada (TC) de imágenes del abdomen y determinar las relaciones entre las dimensiones del hígado y los parámetros antropométricos. La investigación incluyó a 99 pacientes, 49 hombres y 50 mujeres, que fueron remitidos para TC de abdomen. Medimos la altura corporal (BH) y la masa corporal (BM), y calculamos el índice de masa corporal (IMC) y el área de superficie corporal (BSA). Además, en las imágenes de TC medimos los diámetros hepáticos anteroposterior (AP), laterolateral (LL) y dos craneocaudales (uno a nivel de la línea medioclavicular - CCmcl, y el diámetro máximo - CCmax). El volumen del hígado (VI) se calculó con una fórmula. Nuestros resultados mostraron que el diámetro AP se correlacionó positivamente con BSA (r = 0,30) en mujeres. El diámetro de LL se correlacionó positivamente con BH (r=0,43) y BSA (0,31) en hombres. El diámetro CCmcl se correlacionó positivamente con BH (r=0,33), BM (r=0,31) y BSA (r=0,34) en hombres, mientras que en mujeres se correlacionó solo con BH (r=0,38). El diámetro CCmax se correlacionó positivamente con BH (r=0,33) y BSA (r=0,33) en hombres. El VI se correlacionó positivamente con BH y BSA tanto en hombres (r=0,36, r=0,33, respectivamente) como en mujeres (r=0,42, r=0,31, respectivamente), y en hombres también con BM (r=0,34). LL, CCmcl, CCmax y LV se correlacionaron negativamente con el envejecimiento en ambos sexos. Después de los 60 años, hubo una disminución en el tamaño de los diámetros LL, CC y LV. Concluimos que las dimensiones del hígado disminuyen con la edad, independientemente del sexo, en la extensión de los diámetros LL y CC que están relacionados con el tamaño de los parámetros corporales, por lo que para una evaluación precisa del tamaño del hígado se debe medir LV como BH, BM y BSA.

Association between telomere length in the DNA of peripheral blood leukocytes and the propofol dose in anesthesia induction: an observational study

Abstract Introduction: Propofol is a widely used anesthetic and its dose is closely related to aging. Telomere length (TL) is a unique heritable trait, and emerging as a biomarker of aging, health and disease. Telomerase RNA component (TERC) plays an important role in maintaining TL. We proposed a hypothesis that propofol dose in general anesthesia can be predicted by measuring TL before operation, which greatly reduced the risk of anesthesia, especially the elderly. Methods: The association between the propofol dose in anesthesia induction and: TL in the DNA of peripheral blood leukocytes; body weight; sex; difference of the Bispectral Index (BIS) before and after anesthesia induction in patients was evaluated by multivariable linear regression analyses. The mutation at the 5'end or 3'end of TERC was detected. We recruited 100 patients of elective surgery. Results: We found that propofol dose in anesthesia induction was clearly correlated significantly with TL (r = 0.78, p < 0.001), body weight (r = 0.84, p = 0.004), sex (r = 0.83, p= 0.84, p = 0.004), sex (r = 0.83, p = 0.004), and difference of BIS before and after anesthesia induction (r = 0.85, p = 0.029). By comparing the absolute values of standardized regression coefficients (0.58, 0.21, 0.19, and 0.12) of the four variables, it can be seen that TL contributes the most to the propofol dose in anesthesia induction. However, the mutation at the 5' end or 3' end of TERC was not found. Conclusions: These findings provide preliminary evidence that the propofol dose in anesthesia induction was clearly correlated with genetically determined TL. TL may be a promising predictor of the propofol dose, which is beneficial to improve the safety of anesthesia and reduce perioperative complications.

Anthropometric Similarities and Differences in Children of Aymara and Non-Aymara Descent in Northern Chile: Implications for Health and Development / Similitudes y Diferencias Antropométricas en Niños de Etnia Aymara y Ascendencia No-Aymara en el Norte de Chile: Implicaciones para la Salud y el Desarrollo

SUMMARY: This study aims to assess the nutritional status of Aymara and non-Aymara children in Arica, comparing anthropometric measurements of boys and girls aged 4-10 to inform interventions for child health and development. We conducted a non-experimental, quantitative, cross-sectional study in Arica, Chile. The sample included 458 children, with equal representation of Aymara and non-Aymara children from diverse socioeconomic backgrounds. Trained research assistants collected anthropometric data using standardized techniques. IBM SPSS statistical software was used for data analysis, including Student's t-test and the Levene test. Both Aymara and non-Aymara children showed high prevalence of overweight and obesity. Boys had a meso-endomorph somatotype, while girls had an endomorph somatotype. Among 8-year-olds, non-Aymara children had a slightly higher mean body weight (35.87, SD 4.50) compared to Aymara children (32.27, SD 4.31), but the difference was not statistically significant (p>0.05). However, 10-year-old Aymara girls had a significantly higher mean body mass index (22.34, SD 4.21) than non-Aymara girls (20.10, SD 3.58) (p=0.05). Regarding body fat percentage, 10-year- old non-Aymara girls had a slightly higher mean (31.01, SD 5.64) than Aymara girls (26.12, SD 5.63), but the difference was not statistically significant (p>0.05). The study found high levels of overweight and obesity in children from northern Chile, increasing with age for both Aymara and non-Aymara groups. The somatotype patterns were consistent across both groups. Although the differences between Aymara and non-Aymara children were not statistically significant, the Aymara group showed slightly higher levels of overweight and obesity. Further research with a larger sample size is needed to confirm these results and identify potential trends. Efforts should focus on promoting healthy nutrition and physical activity to address the growing problem of overweight and obesity in this region.

Este estudio tiene como objetivo evaluar el estado nutricional de los niños Aymaras y no Aymaras en Arica, comparando las medidas antropométricas de niños y niñas de 4 a 10 años para informar las intervenciones para la salud y el desarrollo infantil. Realizamos un estudio no experimental, cuantitativo, de corte transversal en Arica, Chile. La muestra incluyó a 458 niños, con igual representación de niños Aymaras y no Aymaras de diversos estratos socioeconómicos. Asistentes de investigación capacitados recolectaron datos antropométricos utilizando técnicas estandarizadas. Se utilizó el software estadístico IBM SPSS para el análisis de datos, incluyendo la prueba t de Student y la prueba de Levene. Tanto los niños Aymaras como los no Aymaras presentaron una alta prevalencia de sobrepeso y obesidad. Los niños tenían un somatotipo meso-endomorfo, mientras que las niñas tenían un somatotipo endomorfo. Entre los niños de 8 años, los niños no Aymaras tenían un peso corporal medio ligeramente superior (35,87, DE 4,50) en comparación con los niños Aymaras (32,27, DE 4,31), pero la diferencia no fue estadísticamente significativa (p>0,05). Sin embargo, las niñas Aymaras de 10 años tenían un índice de masa corporal medio significativamente mayor (22,34, SD 4,21) que las niñas no Aymaras (20,10, SD 3,58) (p=0,05). En cuanto al porcentaje de grasa corporal, las niñas no Aymaras de 10 años tuvieron una media ligeramente superior (31,01, DE 5,64) que las niñas Aymaras (26,12, DE 5,63), pero la diferencia no fue estadísticamente significativa (p>0,05). El estudio encontró altos niveles de sobrepeso y obesidad en niños del norte de Chile, aumentando con la edad tanto para los grupos Aymaras como para los no Aymaras. Los patrones de somatotipo fueron consistentes en ambos grupos. Aunque las diferencias entre los niños Aymaras y no Aymaras no fueron estadísticamente significativas, el grupo Aymara mostró niveles ligeramente más altos de sobrepeso y obesidad. Se necesita más investigación con un tamaño de muestra más grande para confirmar estos resultados e identificar tendencias potenciales. Los esfuerzos deben centrarse en promover una nutrición saludable y la actividad física para abordar el creciente problema del sobrepeso y la obesidad en esta región.

Estado nutricio de niños del noroeste de México pre y post pandemia por COVID-19 / Nutritional status of children in northwest Mexico pre and post COVID-19 pandemic

Introducción. Mundialmente se observaron consecuencias negativas en la salud por el aislamiento social durante la pandemia de COVID-19; el sobrepeso y la obesidad mostraron tendencias crecientes. Objetivo. Analizar los cambios en el sobrepeso, obesidad y alimentación de escolares del noroeste de México antes y después del aislamiento por COVID-19. Materiales y métodos. Se colectaron y analizaron el peso, talla y circunferencia de cintura de escolares del noroeste de México pre y post pandemia por COVID 19 (n=479 y n=820). Además, se analizaron los cambios en la alimentación en una submuestra de 203 y 179 escolares pre y post pandemia, respectivamente. Resultados. La edad promedio de los escolares en 2019 fue 8,9 ±1,75 y en el 2022 de 9,1 ± 1,54 años. Se observó un aumento de 6,2 puntos porcentuales en la prevalencia de sobrepeso y obesidad y diferencias en la distribución de las categorías del estado nutricio (p=0,049) entre los dos periodos. También, se observaron cambios en la adiposidad central con un aumento de 3 centímetros en la circunferencia de cintura (p=0,001; 62,6 y 65,6 cm). El índice de alimentación saludable (IAS) mostró una alimentación poco saludable durante los dos periodos. Conclusiones. El aumento en las prevalencias de sobrepeso y obesidad, así como de obesidad central durante la emergencia epidemiológica, indicaron un deterioro del estado nutricio de los escolares, que coincide con los reportes en poblaciones a nivel mundial y en Latinoamérica; los resultados resultan preocupantes dada la problemática antes de la emergencia(AU)

Introduction. Negative health consequences due to social isolation during the COVID-19 pandemic were observed worldwide; overweight and obesity showed increasing trends. Objective. To analyze the changes in overweight, obesity and diet of schoolchildren in northwest Mexico before and after lockdown due to COVID-19. Materials and methods. Weight, height, and waist circumference of schoolchildren (n=479 pre-pandemic and n=820 post-pandemic) were collected in public schools located in medium to high marginalization neighborhoods. In the same periods dietary data was collected from a subsample of 203 and 179 schoolchildren, respectively. Results. The average age of schoolchildren in 2019 was 8,9 ±1,75 and 9,1 ± 1,54 in 2022. An increase in percentage of 6,2 was observed in the overweight plus obesity prevalence and a significant difference in the distribution of nutritional status (p=0,049) between the two periods. In addition, changes in central adiposity were observed, with an increase of 3 centimeters in waist circumference (p=0,001; 62,6 and 65,6 cm). The healthy eating index (HAI) classified the diet of schoolchildren as unhealthy during both periods. Conclusions. The increase in the prevalence of overweight and obesity, as well as central adiposity is worrying given that they were already a health problem before the COVID 19 confinement(AU)

Perfil antropométrico de judocas de elite argentinos / Anthropometric profile of Argentine elite Judokas

Introducción: el judo es un deporte de combate cuya clasificación competitiva depende del peso corporal, lo cual está destinado a promover competencias parejas y equitativas en cuanto a capacidades físicas se refiere. El avance de las ciencias del entrenamiento y de la nutrición deportiva generó una mejora de la composición corporal en los deportistas, redundando en un aumento de los valores de masa muscular y en una disminución de la masa adiposa. El objetivo del estudio fue estimar la composición corporal de las personas que practican Judo participantes del campeonato nacional argentino del año 2021. Materiales y métodos: se realizó un estudio transversal descriptivo a los/as participantes del campeonato nacional de judo del año 2021. Fueron evaluados un total de 183 judocas (51 mujeres y 132 varones), según el protocolo de medición de la ISAK. Para la determinación de la composición corporal se utilizó el método de fraccionamiento anatómico de cinco componentes de Kerry Ross. Resultados: el promedio de las judocas fue de 30,5% para la masa adiposa y de 44,5% para la masa muscular; no se encontraron diferencias estadísticamente significativas en los distintos grupos de edad. Los varones obtuvieron un valor promedio de 22,4% para la masa adiposa y 49,1%para la masa muscular; en este caso se encontraron diferencias significativas entre los distintos grupos de edad, tanto para la masa adiposa como para la muscular y la masa ósea. Discusión: se necesitan más estudios con grupos estratificados en función de la división de peso corporal para poder establecer patrones de referencia específicos para el trabajo en ciencias del ejercicio y la nutrición. Conclusión: la optimización de la composición corporal de las personas que practican Judo redunda positivamente en la mejora de las capacidades físicas específicas. Los datos presentados constituyen una referencia específica de utilidad para la evaluación y seguimiento de las personas que practican Judo. La estratificación por grupos etarios y división de peso corporal permitirá delimitar mejor aún los valores de cada categoría de edad. Se considera relevante vincular la composición corporal con las pruebas físicas y los resultados deportivos, a fin de establecer un mejor perfil del judoca argentino. (AU)

Introduction: judo is a combat sport whose competitive classification depends on body weight, intended to promote even and equitable compe-titions in terms of physical abilities. Advances in the sciences of training and sports nutrition have led to an improvement in the body composition of athletes, resulting in an increase in muscle mass and a decrease in adipose mass. This study aimed to estimate the body composition of judo fighters participating in the 2021 Argentine National Championship. Materials and methods: we conducted a descriptive cross-sectional study of participants of the 2021 national judo championship. We evaluated 183 judokas (51 women and 132 men) applying the ISAK measurement protocol. We used the Kerry Ross five-component fractional anatomic method to determine body composition. Results: the average for female judo players was 30.5% for adipose mass and 44.5% for muscle mass; we found no statistically significant diffe-rences in the different age groups. The average value for males was 22.4% for adipose mass and 49.1% for muscle mass; in this case, we found significant differences between the different age groups for adipose mass and muscle and bone mass. Discussion: there is a need for more studies with stratified groups according to body weight division to establish specific reference standards for the work in exercise science and nutrition. Conclusion: the optimization of the body composition of female and male judo players has a positive effect on the improvement of specific physical capacities. The data presented constitute a relevant reference for the evaluation and follow-up of judokas. The stratification by age groups and body weight division will allow a better delimitation of the values of each age category. Linking body composition with physical tests and results is considered relevant to obtain a better profile of the Argentinian judo player. (AU)

Salud física, calidad de vida y bienestar psicológico de trabajadores(as) universitarios chilenos en tiempos de COVID-19 / Physical health, quality of life and psychological well-being of workers in a Chilean university community in times of COVID-19

Introducción: COVID-19 no solamente tuvo efectos en la salud física de las personas, se sumó a una serie de otras problemáticas que afectarán la salud de las personas a mediano y largo plazo. El objetivo de la investigación fue identificar la percepción del nivel de actividad física, calidad de vida y bienestar psicológico de trabajadores y trabajadoras de una comunidad universitaria chilena durante la pandemia COVID-19. Se aplicó una encuesta online durante los meses de junio y julio de 2022 a285 sujetos, 65% mujeres y 35% hombres, de estamentos, administrativo y académicos (64% y 36% respectivamente). Los resultados indican que un 58% disminuyó su actividad física durante la pandemia en el 2020 y un 51% durante el año 2021. Un 52% señala que aumentó de peso corporal durante el 2020 y un 46% durante el 2021. En relación a su bienestar psicológico, un 49% percibe que empeoró durante el año 2020 y un 36% declara lo mismo para el año 2021.Conclusiones: La pandemia por COVID-19, generó efectos negativos en la actividad física, calidad de vida y bienestar psicológico de las personas. El desafío para las instituciones de educación superior es desarrollar programas para fomentar la promoción y prevención en salud para la comunidad universitaria en un contexto postpandémico(AU)

Introduction: COVID-19 not only had effects on people's physical health, it added to a series of other problems that will affect people's health in the medium and long term. The objective was to identify the perception of the level of physical activity, quality of life and psychological wellbeing of workers in a Chilean university community during the COVID-19 pandemic. The study was developed under a quantitative paradigm, it was descriptive cross-sectional. An online survey was applied during the months of June and July 2022 to 285 subjects, 65% women and 35% men, from estates, administrative and academic (64% and 36% respectively). The results indicate that 58% decreased their physical activity during the pandemic in 2020 and 51% during 2021. 52% say they increased body weight during 2020 and 46% during 2021. In relation to their psychological well-being, 49% perceive that it worsened during the year 2020, and 36% declare the same for the year 2021.Conclusions: The COVID-19 pandemic generated negative effects on people's physical activity, quality of life and psychological well-being. The challenge for higher education institutions is to develop programs to foster health promotion and prevention for the university community in a post-pandemic context(AU)

Percentiles de peso, talla e índice de masa corporal de escolares de Mendoza. Comparación con la referencia de la Organización Mundial de la Salud / Body mass index, weight, and height percentiles in school-aged children from Mendoza. A comparison with the WHO reference

Introducción. La Organización Mundial de la Salud (OMS) recomienda el uso de tablas de referencia para monitorear el crecimiento y estado nutricional de niños, niñas y adolescentes. El peso corporal, la talla y el índice de masa corporal (IMC) son las variables más utilizadas. El presente trabajo tiene como objetivos estimar los percentiles de peso, talla e IMC de escolares (2009-2011) residentes en el departamento San Rafael (Mendoza) y compararlos con la referencia internacional de la Organización Mundial de la Salud, a fin de establecer su pertinencia para la evaluación del crecimiento y estado nutricional de dicha población. Población y métodos. Se realizó un estudio antropométrico transversal en 3448 escolares de entre 4,00 y 13,49 años de edad. Se utilizó el programa LMS ChartMarker Pro para calcular los valores percentilares de peso/edad, talla/edad e IMC/edad, por sexo y edad, y se compararon con las curvas de la OMS. Además, se calcularon diferencias porcentuales (D%) para estimar las diferencias y su significación estadística mediante prueba de Wilcoxon. Resultados. La población de San Rafael mostró, en varones y mujeres, valores percentilares superiores de peso e IMC (D% ≈7 % y 9 %, respectivamente), y menores de talla (D% ≈0,8 %) que los de la OMS (p <0,05). Conclusión. Las diferencias encontradas alertan sobre el empleo de la referencia OMS en la población escolar de San Rafael, ya que sobreestimaría las prevalencias de sobrepeso, obesidad y desnutrición crónica, y subestimaría la de desnutrición aguda y global. Esta situación resalta la importancia de contar con una referencia local.

Introduction. The World Health Organization (WHO) recommends the use of reference tables to monitor the growth pattern and nutritional status of children and adolescents. Body mass index (BMI), weight, and height are the most commonly used variables. The objective of this study was to estimate the BMI, weight, and height percentiles for school-aged children (2009-2011) living in the department of San Rafael (Mendoza) and compare them to the international World Health Organization reference to establish their relevance for the evaluation of the growth pattern and nutritional status of this population. Population and methods. A cross-sectional anthropometric study was conducted in 3448 school-aged children aged 4.00 to 13.49 years. The LMS ChartMarker Pro software was used to estimate the BMI- for-age, weight-for-age, and height-for-age percentiles, by sex and age, and they were compared with the WHO curves. Besides, percentage differences (%D) were calculated to estimate the differences and their statistical significance using the Wilcoxon test. Results. The population of boys and girls in San Rafael showed higher weight and BMI (%D ≈ 7% and 9%, respectively) percentiles, and lower height (%D ≈ 0.8%) values than WHO reference (p < 0.05). Conclusion. The differences found warn about the use of the WHO reference in the school-aged population of San Rafael since it would overestimate the prevalence of overweight, obesity, and chronic malnutrition and underestimate the prevalence of acute and global malnutrition. This situation highlights the importance of having a local reference resource

Compuestos bioactivos de canela y su efecto en la disminución del síndrome metabólico: revisión sistemática / Bioactive compounds of cinnamon and their effect on decreasing metabolic syndrome: systematic review

Introducción. El síndrome metabólico (SM) aumenta el ingreso hospitalario y el riesgo de desarrollar COVID-19, los fármacos utilizados para su tratamiento ocasionan efectos secundarios por lo que se ha optado por la búsqueda de alternativas terapéuticas a base de compuestos bioactivos contenidos en plantas medicinales. La canela se utiliza como agente terapéutico debido a sus propiedades comprobadas con diversos mecanismos de acción reportados en el tratamiento de varias patologías. Objetivo. Documentar los estudios in vitro, in vivo, estudios clínicos y los mecanismos de acción reportados del efecto de la administración de extractos y polvo de canela en las comorbilidades relacionadas con el SM. Materiales y métodos. Revisión sistemàtica de artículos en bases de datos electrónicas, incluyendo estudios de canela en polvo, extractos acuosos, de acetato de etilo y metanol de la corteza de canela, período de 5 años, excluyendo todo artículo relacionado a su efecto antimicrobiano, antifúngico y aceite de canela. Resultados. Las evidencias de los principales compuestos bioactivos contenidos en la canela validan su potencial en el tratamiento de enfermedades relacionadas al SM, con limitados estudios que indagan en los mecanismos de acción correspondientes a sus actividades biológicas. Conclusiones. Las evidencias de las investigaciones validan su potencial en el tratamiento de estas patologías, debido a sus principales compuestos bioactivos: cinamaldehído, transcinamaldehído, ácido cinámico, eugenol y, antioxidantes del tipo proantocianidinas A y flavonoides, los cuales participan en diversos mecanismos de acción que activan e inhiben enzimas, con efecto hipoglucemiante (quinasa y fosfatasa), antiobesogénico (UPC1), antiinflamatorio (NOS y COX), hipolipemiante (HMG-CoA) y antihipertensivo (ECA)(AU)

Introduction. Metabolic syndrome (MS) increases hospital admission and the risk of developing COVID-19. Due to the side effects caused by the drugs used for its treatment, the search for therapeutic alternatives based on bioactive compounds contained in medicinal plants has been chosen. Cinnamon is used as a therapeutic agent due to its proven properties with various mechanisms of action reported in the treatment of various pathologies. Objective. To document the in vitro and in vivo studies, clinical studies and the mechanisms of action reported on the effect of the administration of cinnamon extracts and powder on comorbidities related to MS. Materials and methods. Systematic review of articles in electronic databases, including studies of cinnamon powder, aqueous extracts, ethyl acetate and methanol from cinnamon bark, over a period of 5 years, excluding all those articles related to its antimicrobial, antifungal and antimicrobial effect. cinnamon oil. Results. The evidence of the main bioactive compounds contained in cinnamon validates its potential in the treatment of diseases related to MS, with limited studies that investigate the mechanisms of action corresponding to its biological activities. Conclusions. Research evidence validates its potential in the treatment of these pathologies, due to its main bioactive compounds: cinnamaldehyde, transcinnamaldehyde, cinnamic acid, eugenol, and antioxidants of the proanthocyanidin A type and flavonoids, which participate in various mechanisms of action that activate and they inhibit enzymes, with hypoglycemic (kinase and phosphatase), antiobesogenic (UPC1), anti-inflammatory (NOS and COX), lipid-lowering (HMG-CoA) and antihypertensive (ACE) effects(AU)

Variaciones morfológicas de la glándula submandibular en ratas obesas por acción de glutamato monosódico / Morphological variations of the submandibular gland in obese rats by the action of monosodium glutamate

Este estudio tuvo como objetivo demostrar la existencia de variaciones morfológicas en el tejido conectivo de la glándula submandibular de ratas obesas expuestas a glutamato monosódico (GMS). Se utilizaron 12 ratas Sprague Dawley machos recién nacidas (6 ratas para el grupo 1, control; 6 ratas para el grupo 2 (GMS), 4 mg/g de glutamato monosódico de peso (5 dosis) mantenidas por 16 semanas respectivamente con una dieta y agua ad libitum. En el estudio se realizó un análisis estereológico e histológico, demostrándose una variación en el tejido conectivo presentando una disminución del volúmen glandular, mayor fibrosis, y disminución de adipocitos a nivel periférico siendo reemplazado por tejido rico en colágeno. Los vasos sanguíneos observados a nivel estereológico no presentan mayores cambios en cuanto a volumen, superficie y área.

SUMMARY: This study aims to demonstrate the existence of morphological variations in the connective tissue of the submandibular gland of obese rats exposed to MSG. Twelve male newborn Sprague Dawley rats were used (6 rats for group 1, control; 6 rats for group 2 (MSG), 4 mg/g of monosodium glutamate of weight (5 doses) maintained for 16 weeks respectively with a diet and water ad libitum. In the study, a stereological and histological analysis was carried out, demonstrating a variation in the connective tissue, presenting a decrease in the glandular volume, greater fibrosis, and a decrease in adipocytes at the peripheral level, being replaced by tissue rich in collagen. Blood cells observed at the stereological level do not present major changes in terms of volume, surface and area, but in the histological study greater vascularization is observed.

Avaliação do consumo alimentar e estado nutricional de crianças menores de dois anos de uma comunidade quilombola / Assessment of food consumption and nutritional status of children under two years of age in a quilombola community / Evaluación del consumo de alimentos y del estado nutricional de niños menores de dos años en una comunidad quilombola

A Vigilância Alimentar e Nutricional é uma das diretrizes da Política Nacional de Alimentação e Nutrição, sendo considerada essencial para a atenção nutrição e monitoramento do estado nutricional e das práticas de consumo alimentar, possibilitando o reconhecimento de alimentos ou comportamentos que se relacionam à alimentação saudável e não saudável para fornecer subsídios para o planejamento e organização do cuidado. O objetivo deste estudo foi avaliar o consumo alimentar e o estado nutricional de crianças de 0 a 23 meses que residem na Comunidade Quilombola de Córrego dos Iús-CE. O percurso metodológico trata-se de um estudo do tipo pesquisa- intervenção, que consistiu em uma tendência das pesquisas participativas que busca compreender a vida de coletividades na sua diversidade qualitativa. Identificamos 11 crianças, menores de dois anos na comunidade. Devido às perdas, às recusas ou à ausência do responsável no domicílio, a amostra estudada foi composta por 9 participantes. A partir da avaliação antropométrica, verificamos os agravos nutricionais mais importantes foram o peso elevado para idade (44.4%), risco de sobrepeso para estatura e obesidade para estatura apresentaram respectivamente (11.1%), sobrepeso para IMC por idade (11.1%). Os achados sugerem que a população estudada esteja passando por uma transição nutricional. Nossa pesquisa nos permitiu perceber que as crianças pertencem as famílias de baixo poder econômico. É necessário a consolidação de políticas públicas para melhorar a saúde da comunidade estudada.

Food and Nutrition Surveillance is one of the guidelines of the National Food and Nutrition Policy, and is considered essential for nutritional care and monitoring of nutritional status and food consumption practices, enabling the recognition of foods or behaviors that are related to healthy eating and unhealthy to provide subsidies for the planning and organization of care. The objective of this study was to evaluate the food consumption and nutritional status of children aged 0 to 23 months living in the Quilombola Community of Córrego dos Iús-CE. The methodological course is a research- intervention type study, which consisted of a trend of participatory research that seeks to understand the life of collectivities in its qualitative diversity. We identified 11 children under two years of age in the community. Due to losses, refusals or the absence of the responsible person at home, the studied sample consisted of 9 participants. From the anthropometric assessment, we verified the most important nutritional problems were high weight for age (44.4%), risk of overweight for height and obesity for height presented respectively (11.1%), overweight for BMI for age (11.1%). The findings suggest that the studied population is going through a nutritional transition. Our research allowed us to realize that children belong to families with low economic power. It is necessary to consolidate public policies to improve the health of the studied community. KEYWORDS: Health Education; Nutritional Status; Group with Ancestors from the African Continent; Public health.

La Vigilancia Alimentaria y Nutricional es una de las directrices de la Política Nacional de Alimentación y Nutrición, y se considera esencial para la atención nutricional y el seguimiento del estado nutricional y las prácticas de consumo de alimentos, lo que permite el reconocimiento de los alimentos o comportamientos que están relacionados con la alimentación saludable y no saludable para proporcionar subsidios para la planificación y organización de la atención. El objetivo de este estudio fue evaluar el consumo de alimentos y el estado nutricional de los niños de 0 a 23 meses que viven en la Comunidad Quilombola de Córrego dos Iús-CE. El curso metodológico es un estudio de tipo investigación-intervención, que consistió en una tendencia de investigación participativa que busca comprender la vida de las colectividades en su diversidad cualitativa. Identificamos 11 niños menores de dos años en la comunidad. Debido a pérdidas, negativas o ausencia del responsable en el domicilio, la muestra estudiada quedó constituida por 9 participantes. A partir de la evaluación antropométrica, verificamos que los problemas nutricionales más importantes fueron peso elevado para la edad (44,4%), riesgo de sobrepeso para la estatura y obesidad para la estatura presentados respectivamente (11,1%), sobrepeso para el IMC para la edad (11,1%). Los hallazgos sugieren que la población estudiada está pasando por una transición nutricional. Nuestra investigación nos permitió constatar que los niños pertenecen a familias de bajo poder económico. Es necesario consolidar políticas públicas para mejorar la salud de la comunidad estudiada.

Atletas de Taekwondo apresentam desequilíbrio muscular entre os músculos flexores e extensores do joelho: achados preliminares / Taekwondo athletes have muscle imbalance between knee flexors and extensions: preliminary findings / Los atletas de Taekwondo presentan un desequilibrio muscular entre los flexores y los extensores de la rodilla: hallazgos preliminares

Durante a prática de taekwondo com movimentos repetitivos, sistematizados e com certa sobrecarga de treino, o indivíduo pode gerar possíveis adaptações orgânicas que resultam em problemas posturais com grandes chances de desencadear desequilíbrio muscular. Objetivo: Verificar a presença de desequilíbrio entre os grupos musculares agonistas e antagonistas da articulação do joelho e entre membros dominantes e não dominantes de praticantes de taekwondo por meio da dinamometria isocinética. Método: Estudo transversal, observacional e descritivo realizado com nove praticantes de taekwondo do sexo masculino. Utilizou-se um dinamômetro isocinético para investigar o pico de torque, pico de torque por peso corporal, trabalho total, potência média, relação agonista/antagonista e índice de fadiga. Os dados dos membros dominante e não dominante foram comparados por meio do teste t-student para amostras pareadas. Foram calculados o intervalo de confiança de 95% da diferença média, o tamanho de efeito e o poder das análises. Resultados: Os músculos extensores dos membros dominante e não dominante apresentaram diferença média significante de 15,49 Nm (IC95% 7,27; 23,70; p=0,002) para pico de torque e de 22,64% (IC95% 11,83; 33,46; p=0,001) para pico de torque por peso corporal a 60°/s, representando tamanho de efeito médio. Conclusão: Os atletas de taekwondo apresentaram maior pico de torque e maior pico de torque por peso corporal dos músculos extensores do joelho a 60º/s no lado dominante. A relação agonista/ antagonista foi inferior a 60% e mais da metade dos atletas apresentaram uma diferença maior que 10% no pico de torque flexor no lado não dominante.

During taekwondo practice with the repetitive motions, systematized and with certain training overload, the person can generate possible organic adaptations that result in postural problems with a great chances of triggering muscle imbalance. Objective: To verify the presence of imbalance between agonist and antagonist muscle groups of knee joint and between dominant and non-dominant limbs through isokinetic dynamometry. Methods: Cross-sectional, observational and descriptive study realized with nine male taekwondo practitioners. An isokinetic dynamometer was used to investigate the peak torque, peak torque by body weight, total work, average power, agonist/antagonist ratio and fatigue index. Data from the dominant and non-dominant limbs were compared by t-student test for pared samples. The 95% confidence interval of the mean difference, the effect size and the power of analyses power were calculated. Results: The extensor muscles of the dominant and non-dominant limbs showed mean difference of 15,49 Nm (IC95% 7,27; 23,70; p=0,002) for peak torque and of 22,64% (IC95% 11,83; 33,46; p=0,001) for peak torque by body weight at 60°/s, representing average effect size. Conclusion: The taekwondo athletes had higher peak torque and higher peak torque by body weight of the knee extensors muscles in the dominant side. The agonist/ antagonist ratio was less than 60% and more than half of the athletes showed a difference greater than 10% in the peak flexor torque on the non-dominant side.

Durante la práctica de taekwondo con los movimientos repetitivos, sistematizados y con cierta sobrecarga de entrenamiento, la persona puede generar posibles adaptaciones orgánicas que deriven en problemas posturales con grandes posibilidades de desencadenar desequilibrios musculares. Objetivo: Verificar la presencia de desequilibrio entre grupos musculares agonistas y antagonistas de la articulación de la rodilla y entre miembros dominantes y no dominantes mediante dinamometría isocinética. Métodos: Estudio transversal, observacional y descriptivo realizado con nueve practicantes masculinos de taekwondo. Se utilizó un dinamómetro isocinético para investigar el par máximo, el par máximo por peso corporal, el trabajo total, la potencia media, la relación agonista/antagonista y el índice de fatiga. Los datos de las extremidades dominantes y no dominantes se compararon mediante la prueba t- student para muestras de pared. Se calcularon el intervalo de confianza del 95% de la diferencia media, el tamaño del efecto y la potencia de los análisis. Resultados: Los músculos extensores de los miembros dominantes y no dominantes mostraron una diferencia media de 15,49 Nm (IC95% 7,27; 23,70; p=0,002) para el par máximo y de 22,64% (IC95% 11,83; 33,46; p=0,001) para el par máximo por peso corporal a 60°/s, lo que representa el tamaño medio del efecto. Conclusiones: Los atletas de taekwondo presentaron un mayor par máximo y un mayor par máximo por peso corporal de los músculos extensores de la rodilla en el lado dominante. La relación agonista/antagonista fue inferior al 60% y más de la mitad de los atletas mostraron una diferencia superior al 10% en el pico de par flexor en el lado no dominante.

Mathematical models to describe the growth curves of Vietnamese Ri chicken / Modelos matemáticos para descrever as curvas de crescimento do frango Ri vietnamita

Abstract Ri chicken is the most popular backyard chicken breed in Vietnam, but little is known about the growth curve of this breed. This study compared the performances of models with three parameters (Gompertz, Brody, and Logistic) and models containing four parameters (Richards, Bridges, and Janoschek) for describing the growth of Ri chicken. The bodyweight of Ri chicken was recorded weekly from week 1 to week 19. Growth models were fitted using minpack.lm package in R software and Akaike's information criterion (AIC), Bayesian information criterion (BIC), and root mean square error (RMSE) were used for model comparison. Based on these criteria, the models having four parameters showed better performance than the ones with three parameters, and the Richards model was the best one for males and females. The lowest and highest value of asymmetric weights (α) were obtained by Bridges and Brody models for each of sexes, respectively. Age and weight estimated by the Richard model were 8.46 and 7.51 weeks and 696.88 and 487.58 g for males and for females, respectively. Differences in the growth curves were observed between males and female chicken. Overall, the results suggested using the Richards model for describing the growth curve of Ri chickens. Further studies on the genetics and genomics of the obtained growth parameters are required before using them for the genetic improvement of Ri chickens.

Resumo O frango Ri é a raça de frango de quintal mais popular do Vietnã, mas pouco se sabe sobre a curva de crescimento dessa raça. Este estudo comparou o desempenho de modelos com três parâmetros (Gompertz, Brody e Logistic) e modelos contendo quatro parâmetros (Richards, Bridges e Janoschek) para descrever o crescimento do frango Ri. O peso corporal do frango Ri foi registrado semanalmente da semana 1 à semana 19. Os modelos de crescimento foram ajustados usando o pacote minpack.lm no software R e o critério de informação de Akaike (AIC); critério de informação bayesiano (BIC) e erro quadrático médio (RMSE) foram usados ​​para comparação de modelos. Com base nesses critérios, os modelos com quatro parâmetros apresentaram melhor desempenho do que os com três parâmetros, sendo o modelo de Richards o melhor para homens e mulheres. O menor e o maior valor dos pesos assimétricos (α) foram obtidos pelos modelos Bridges e Brody para cada um dos sexos, respectivamente. A idade e o peso estimados pelo modelo de Richard foram de 8,46 e 7,51 semanas e 696,88 e 487,58 g para homens e mulheres, respectivamente. Diferenças nas curvas de crescimento foram observadas entre frangos machos e fêmeas. No geral, os resultados sugeriram o uso do modelo de Richards para descrever a curva de crescimento de frangos Ri. Mais estudos sobre a genética e genômica dos parâmetros de crescimento obtidos são necessários antes de usá-los para o melhoramento genético de frangos Ri.

An improved 4-vessel intermittent occlusion method for establishing rat models of global cerebral ischemia-reperfusion injury / 南方医科大学学报

OBJECTIVE@#To improve the classical 4-vessel occlusion (4VO) model established by Pulsinelli and Brierley.@*METHODS@#Thirty-two male SD rats were randomized into sham operation group, I4VO-Con10 group, I4VO-Int10 group and I4VO-Int15 group. The sham surgery group underwent exposure of the bilateral vertebral arteries and carotid arteries without occlusion to block blood flow. The I4VO-Con10 group experienced continuous ischemia by occluding the bilateral vertebral arteries and carotid arteries for 10 minutes followed by reperfusion for 24 hours. The I4VO-Int10 and I4VO-Int15 groups were subjected to intermittent ischemia. The I4VO- Int10 group underwent 5 minutes of ischemia, followed by 5 minutes of reperfusion and another 5 minutes of ischemia, and then reperfusion for 24 hours. The I4VO-Int15 group experienced 5 minutes of ischemia followed by two cycles of 5 minutes of reperfusion and 5 minutes of ischemia, and then reperfusion for 24 hours. The regional cerebral blood flow (rCBF) was monitored with laser Doppler scanning, and survival of the rats was observed. HE staining was used to observe hippocampal pathologies to determine the optimal method for modeling. Another 48 rats were randomized into 6 groups, including a sham operation group and 5 model groups established using the optimal method. The 5 I4VO model groups were further divided based on the reperfusion time points (1, 3, 7, 14, and 28 days) into I4VO-D1, I4VO-D3, I4VO-D7, I4VO- D14, and I4VO- D28 groups. Body weight changes and survival of the rats were recorded. HE staining was used to observe morphological changes in the hippocampal, retinal and optic tract tissues. The Y-maze test and light/dark box test were used to evaluate cognitive and visual functions of the rats in I4VO-D28 group.@*RESULTS@#Occlusion for 5 min for 3 times at the interval of 5 min was the optimal method for 4VO modeling. In the latter 48 rats, the body weight was significantly lower than that of the sham-operated rats at 1, 3, 7, 14 and 28 days after modeling without significant difference in survival rate among the groups. The rats with intermittent vessel occlusion exhibited progressive deterioration of hippocampal neuronal injury and neuronal loss. Cognitive impairment was observed in the rats in I4VO-D28 group, but no obvious ischemic injury of the retina or the optic tract was detected.@*CONCLUSION@#The improved 4VO model can successfully mimic the main pathological processes of global cerebral ischemia-reperfusion injury without causing visual impairment in rats.

Molecular pathological mechanism of liver metabolic disorder in mice with severe spinal muscular atrophy / 南方医科大学学报

OBJECTIVE@#To explore the molecular pathological mechanism of liver metabolic disorder in severe spinal muscular atrophy (SMA).@*METHODS@#The transgenic mice with type Ⅰ SMA (Smn-/- SMN20tg/2tg) and littermate control mice (Smn+/- SMN20tg/2tg) were observed for milk suckling behavior and body weight changes after birth. The mice with type Ⅰ SMA mice were given an intraperitoneal injection of 20% glucose solution or saline (15 μL/12 h), and their survival time was recorded. GO enrichment analysis was performed using the RNA-Seq data of the liver of type Ⅰ SMA and littermate control mice, and the results were verified using quantitative real-time PCR. Bisulfite sequencing was performed to examine CpG island methylation level in Fasn gene promoter region in the liver of the neonatal mice.@*RESULTS@#The neonatal mice with type Ⅰ SMA showed normal milk suckling behavior but had lower body weight than the littermate control mice on the second day after birth. Intraperitoneal injection of glucose solution every 12 h significantly improved the median survival time of type Ⅰ SMA mice from 9±1.3 to 11± 1.5 days (P < 0.05). Analysis of the RNA-Seq data of the liver showed that the expression of the target genes of PPARα related to lipid metabolism and mitochondrial β oxidation were down-regulated in the liver of type Ⅰ SMA mice. Type Ⅰ SMA mice had higher methylation level of the Fasn promoter region in the liver than the littermate control mice (76.44% vs 58.67%). In primary cultures of hepatocytes from type Ⅰ SMA mice, treatment with 5-AzaC significantly up-regulated the expressions of the genes related to lipid metabolism by over 1 fold (P < 0.01).@*CONCLUSION@#Type Ⅰ SMA mice have liver metabolic disorder, and the down-regulation of the target genes of PPARα related to lipid and glucose metabolism due to persistent DNA methylation contributes to the progression of SMA.

Improvement situation on indexes of the zebrafish disease model of non-alcoholic fatty liver disease with FGF21 analogues / 中华肝脏病杂志

Objective: To detect the therapeutic efficacy of FGF21 analogues on the zebrafish model of non-alcoholic fatty liver disease. Methods: A zebrafish model of non-alcoholic fatty liver disease was established by providing the normal diet fed to wild-type zebrafish three times daily. PF-05231023 was administered exogenously at a final concentration of 0.5 μmol/L. Body length, body weight, triglycerides, and other indexes were measured after 20 days. Pathological changes were evaluated in liver tissue sections by HE staining. Quantitative PCR was used to identify expressional changes in genes related to lipid metabolism, endoplasmic reticulum stress, and inflammation. Results: QPCR and immunofluorescence staining results showed that FGF21 was highly expressed in the zebrafish model group. The addition of the FGF21 analogue PF-05231023 significantly reduced the body length and body weight (P < 0.01), and the triglyceride content (P < 0.05) in the zebrafish model group. The liver HE staining results showed that PF-05231023 had alleviated the large and tiny bullae fat, lesions, and others in the zebrafish model group. The quantitative PCR results demonstrated that PF-05231023 reduced the expression of lipogenic factors (P < 0.01), inflammatory-related factors (P < 0.001), and genes related to endoplasmic reticulum stress (P < 0.05), but raised lipid-oxidation-related factors (P < 0.05) in the zebrafish model group. The addition of PF-05231023 reduced oleic acid-induced lipid and triglyceride levels in HepG2 cells. Conclusion: FGF21 analogue addition can improve indexes in the zebrafish disease model of non-alcoholic fatty liver disease.

Effect of diosgenin on mTOR/FASN/HIF-1α/VEGFA expression in rats with non-alcoholic fatty liver disease / 中国中药杂志

The present study aimed to investigate the effect of diosgenin on mammalian target of rapamycin(mTOR), fatty acid synthase(FASN), hypoxia inducible factor-1α(HIF-1α), and vascular endothelial growth factor A(VEGFA) expression in liver tissues of rats with non-alcoholic fatty liver disease(NAFLD) and explore the mechanism of diosgenin on lipogenesis and inflammation in NAFLD. Forty male SD rats were divided into a normal group(n=8) fed on the normal diet and an experimental group(n=32) fed on the high-fat diet(HFD) for the induction of the NAFLD model. After modeling, the rats in the experimental group were randomly divided into an HFD group, a low-dose diosgenin group(150 mg·kg~(-1)·d~(-1)), a high-dose diosgenin group(300 mg·kg~(-1)·d~(-1)), and a simvastatin group(4 mg·kg~(-1)·d~(-1)), with eight rats in each group. The drugs were continuously given by gavage for eight weeks. The levels of triglyceride(TG), total cholesterol(TC), low-density lipoprotein cholesterol(LDL-C), alanine transaminase(ALT), and aspartate transaminase(AST) in the serum were detected by the biochemical method. The content of TG and TC in the liver was detected by the enzyme method. Enzyme-linked immunosorbent assay(ELISA) was used to measure interleukin 1β(IL-1β) and tumor necrosis factor α(TNF-α) in the serum. Lipid accumulation in the liver was detected by oil red O staining. Pathological changes of liver tissues were detected by hematoxylin-eosin(HE) staining. The mRNA and protein expression levels of mTOR, FASN, HIF-1α, and VEGFA in the liver of rats were detected by real-time fluorescence-based quantitative polymerase chain reaction(PCR) and Western blot, respectively. Compared with the normal group, the HFD group showed elevated body weight and levels of TG, TC, LDL-C, ALT, AST, IL-1β, and TNF-α(P<0.01), increased lipid accumulation in the liver(P<0.01), obvious liver steatosis, up-regulated mRNA expression levels of mTOR, FASN, HIF-1α, and VEGFA(P<0.01), and increased protein expression levels of p-mTOR, FASN, HIF-1α, and VEGFA(P<0.01). Compared with the HFD group, the groups with drug treatment showed lowered body weight and levels of TG, TC, LDL-C, ALT, AST, IL-1β, and TNF-α(P<0.05, P<0.01), reduced lipid accumulation in the liver(P<0.01), improved liver steatosis, decreased mRNA expression levels of mTOR, FASN, HIF-1α, and VEGFA(P<0.05, P<0.01), and declining protein expression levels of p-mTOR, FASN, HIF-1α, and VEGFA(P<0.01). The therapeutic effect of the high-dose diosgenin group was superior to that of the low-dose diosgenin group and the simvastatin group. Diosgenin may reduce liver lipid synthesis and inflammation and potentiate by down-regulating the mTOR, FASN, HIF-1α, and VEGFA expression, playing an active role in preventing and treating NAFLD.

Punicalagin inhibits hepatic lipid deposition in obese mice via AMPK/ACC pathway / 中国中药杂志

Hepatic lipid deposition is one of the basic manifestations of obesity, and nowadays pharmacological treatment is the most important tool. Punicalagin(PU), a polyphenol derived from pomegranate peel, is a potential anti-obesity substance. In this study, 60 C57BL/6J mice were randomly divided into a normal group and a model group. After establishing a model of simple obesity with a high-fat diet for 12 weeks, the successfully established rat models of obesity were then regrouped into a model group, an orlistat group, a PU low-dose group, a PU medium-dose group, and a PU high-dose group. The normal group was kept on routine diet and other groups continued to feed the high-fat diet. The body weight and food intake were measured and recorded weekly. After 8 weeks, the levels of the four lipids in the serum of each group of mice were determined by an automatic biochemical instrument. Oral glucose tole-rance and intraperitoneal insulin sensitivity were tested. Hemoxylin-eosin(HE) staining was applied to observe the hepatic and adipose tissues. The mRNA expression levels of peroxisome proliferators-activated receptor γ(PPARγ) and C/EBPα were determined by real-time quantitative polymerase chain reaction(Q-PCR), and the mRNA and protein expression levels of adenosine 5'-monophosphate-activated protein kinase(AMPK), anterior cingulate cortex(ACC), and carnitine palmitoyltransferase 1A(CPT1A) were determined by Western blot. Finally, the body mass, Lee's index, serum total glyceride(TG), serum total cholesterol(TC), and low-density lipoprotein cholesterol(LDL-C) levels were significantly higher and high-density lipoprotein cholesterol(HDL-C) levels were significantly lower in the model group as compared with the normal group. The fat deposition in the liver was significantly increased. The mRNA expression levels of hepatic PPARγ and C/EBPα and the protein expression level of ACC were increased, while the mRNA and protein expression levels of CPT-1α(CPT1A) and AMPK were decreased. After PU treatment, the above indexes of obese mice were reversed. In conclusion, PU can decrease the body weight of obese mice and control their food intake. It also plays a role in the regulation of lipid metabolism and glycometabolism metabolism, which can significantly improve hepatic fat deposition. Mechanistically, PU may regulate liver lipid deposition in obese mice by down-regulating lipid synthesis and up-regulating lipolysis through activation of the AMPK/ACC pathway.